Investigation and analysis of myopia and ocular biological parameters of primary and secondary school students in Urumqi / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To investigate the differences of myopia and ocular biological parameters of primary and middle school students in Urumqi.METHODS: A cross-sectional survey. A total of 2 495 primary and middle school students aged 7 to 18 from 4 schools in Urumqi were selected by judgemental sampling from September 2021 to November 2021 for relevant eye examination. The differences of ocular biological parameters and refractive status of students of different ages, genders and nationalities were compared, and the correlation between spherical equivalent(SE)and ocular biological parameters was analyzed.RESULTS: The detection rate of poor vision among the students was 80.36%, the detection rate of myopia was 55.91%, and the detection rate of astigmatism was 42.96%, among which the detection rate of low myopia was 63.80%, the detection rate of moderate myopia was 27.60%, and the detection rate of high myopia was 8.60%. There were significant differences in the detection rate of poor vision, myopia, astigmatism, SE and some ocular biological parameters among students of different ages and nationalities(all P<0.05). Among them, the detection rate of myopia, astigmatism and poor vision among Uygur and Kazakh ethnic groups was significantly lower than that of Han. The detection rate of poor vision and myopia among boys was lower than that among girls, while the detection rate of astigmatism was higher than that of girls. Spearman correlation analysis showed that axis length of eye, axial length to axial ratio, anterior chamber depth, and pupil diameter were negatively correlated with SE(rs=-0.664, -0.724, -0.320, -0.086, all P<0.001), and lens thickness was positively correlated with SE(rs=0.147, P<0.001).CONCLUSION: The prevalence of myopia among primary and secondary school students in Urumqi is high, and there are differences in the distribution of ocular biological parameters among children and adolescents of different ages and ethnicities.

Myopia and hyperopia reserve of Uyghur children aged 6～12 years in Yecheng county of Xinjiang / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To investigate the myopia and hyperopia reserve among Uyghur children aged 6～12 in Yecheng county of Xinjiang.METHODS: In a cross-sectional study, a total of 50 151 Uyghur students aged 6～12 years from Yecheng county of Xinjiang underwent comprehensive vision screening, including automatic computer optometry, indoor remote light box E-word standard logarithmic visual acuity chart(GB 11533), to measure the naked eye distant vision and corrected vision with lens.RESULTS: From October 2021 to January 2022, the spherical equivalent(SE)of 6-12 year old Uyghur myopic children was -0.875(-1.625, -0.625)D, and the detection rate of myopia was 6.75%(3384/50151)in Yecheng county of Xinjiang. The SE of the myopic children aged 6～12 was -0.75(-1.125, -0.625)D, -0.75(-1.125, -0.625)D, -0.875(-1.125, -0.625)D, -0.875(-1.375, -0.625)D, -0.875(-1.6, -0.625)D, -0.875(-1.625, -0.625)D, -1.25(-2.125, -0.75)D, respectively. The SE of non-myopic children aged 6～12 was +0.25(-0.125, +0.5)D, and the detection rate of insufficient hyperopia reserve was 75.51%. The SE of the non-myopic children aged 6～12 was +0.25(-0.125, +0.5)D, +0.25(0, +0.5)D, +0.125(-0.125, +0.5)D, +0.25(-0.125, +0.5)D, +0.125(-0.125, +0.5)D, +0.125(-0.125, +0.5)D, +0.25(-0.125, +0.625)D, respectively. The detection rates of myopia children aged 6～12 were 6.78%, 5.64%, 5.72%, 5.36%, 5.01%, 6.82%, 12.14%, respectively. The detection rates of insufficient hyperopia reserve children aged 6～12 were 91.67%, 92.04%, 92.91%, 83.85%, 68.89%, 54.01%, 26.71%, respectively. The detection rate of insufficient hyperopia reserve in non-myopic girls aged 9～12 years old was higher than that in boys(χ2=8.94, 19.99, 19.91 and 39.11, all P&#x003C;0. 05); The detection rate of myopia in 10～12 year old girls was higher than that in boys(χ2=5.402, 8.493 and 36.9711, all P&#x003C;0. 05).CONCLUSIONS: The prevalence rate of myopia among Uyghur children aged 6～12 years in Yecheng county of Xinjiang was 6.75%, while the detection rate of insufficient hyperopia reserve in non-myopic children reached up to 75.51%. It is predicted that there are a large number of children at high risk of myopia. Therefore, early monitoring of hyperopia reserve in children is vital for myopia prevention and control strategy.

Visual Detection of Vibrio parahaemolyticus using Combined CRISPR/Cas12a and Recombinase Polymerase Amplification / 生物医学与环境科学（英文）

Objective@#To establish an ultra-sensitive, ultra-fast, visible detection method for Vibrio parahaemolyticus (VP) .@*Methods@#We established a new method for detecting the tdh and trh genes of VP using clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 12a (CRISPR/Cas12a) combined with recombinase polymerase amplification and visual detection (CRISPR/Cas12a-VD).@*Results@#CRISPR/Cas12a-VD accurately detected target DNA at concentrations as low as 10 -18 M (single molecule detection) within 30 min without cross-reactivity against other bacteria. When detecting pure cultures of VP, the consistency of results reached 100% compared with real-time PCR. The method accurately analysed pure cultures and spiked shrimp samples at concentrations as low as 10 2 CFU/g.@*Conclusion@#The novel CRISPR/Cas12a-VD method for detecting VP performed better than traditional detection methods, such as real-time PCR, and has great potential for preventing the spread of pathogens.

Investigation and analysis of dry eye condition of residents in Hotan, Xinjiang / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To evaluate the prevalence and potential risk factors of dry eye among residents in Hotan, Xinjiang, China.METHODS: A cross-sectional study was conducted on 6 027 residents aged 18-98 from 105 villages in Hotan, Xinjiang, China from January 2019 to September 2019. The subjective symptoms of dry eye were collected by ocular surface disease index(OSDI)questionnaire, and the objective signs were collected by testing fluorescein breakup time(FBUT)and Schirmer Ⅰ test, so as to analyze the prevalence and risk factors of dry eye.RESULTS: A total of 6 339 subjects aged 18-98 years were recruited from residents in Hotan, Xinjiang, China. Relevant eye examinations and questionnaires were conducted on the subjects, of which 6 027 were valid questionnaires, with an effective recovery rate of 95.08%. The dry eye detection rate of 6 027 residents was 40.37%(2 433/6 027), and the dry eye detection rates of male and female were 36.47%(846/2 320)and 42.81%(1 587/3 707)respectively. The dry eye detection rates of 18-24 years old, 25-34 years old, 35-44 years old, 45-54 years old, 55-64 years old, and ≥65 years old were 13.77%, 15.67%, 33.31%, 46.35%, 47.65%, 53.50%, respectively. According to the severity of dry eye, they were divided into mild dry eye, moderate dry eye and severe dry eye, and their constituent ratios were 80.11%, 19.03% and 0.86%, respectively.CONCLUSION: The prevalence of dry eye in the study population was 40.37%. The prevalence of dry eye in female was higher than that in male, and the prevalence of dry eye increased with the increase of age. Older age and female are the risk factors for dry eye.

Practice of PBL teaching rounds in standardized residency training of anesthesiology / 中华医学教育探索杂志

Teaching rounds are an important part of hospitals at all levels to carry out clinical teaching work and improve medical quality. Taken anesthesia-related knowledge as the center, based on the patient's pathophysiology and anesthesia physiological changes, our department asked the core questions such as the impact of the operation method and anesthesia plan, then students answered, and finally closed the loop after the case. This process is called problem-based learning (PBL) teaching rounds. It is found that the teaching rounds can help improve the ability of anesthesiologists to solve problems, learn independently, and learn throughout their lives, thereby improving the teaching quality of standardized residency training of anesthesiology.

The impact of obstructive sleep apnea hypopnea syndrome on the perioperative and long-term outcome in patients with Stanford type A aortic dissection / 中华耳鼻咽喉头颈外科杂志

Objective: To confirm the impact of obstructive sleep apnea hypopnea syndrome (OSAHS) on perioperative and long-term outcome in patients with Stanford type A aortic dissection. Methods: From June 2010 to July 2017, the clinical data of 91 patients with Stanford type A aortic dissection were analyzed. Among them, 51 patients with OSAHS were included in the study group and 40 patients without OSAHS were included in the control group. After 36 months follow-up, all-cause death was regarded as the end event. The clinical baseline data, perioperative period and 36 months survival rate of the two groups were compared. Kanplan-Meier method was used to describe the 36 month survival curve of the two groups. Cox proportional risk model was used to evaluate the risk ratio (HR) and 95%CI of 36 month survival rate. Results: The mortality rate during hospitalization was 5.9% (3 cases) in the study group and 5.0% (2 cases) in the control group, and the difference was not statistically significant (χ~2=0.03, P>0.05). The actual follow-up was (36.2±1.5) months, 88 cases were followed up and 3 cases were lost. The all cause mortality rate of 36 months was 27.5% (14/51)in the study group and 10.0%(4/40) in the control group, the difference was statistically significant (χ~2=4.30, P<0.05).By Cox proportional risk model analysis, 36 months after operation, the study group was compared with the control group after adjusting for age, male, bicuspid of aortic valve, chronic obstructive pulmonary disease, anemia, preoperative pericardial tamponade, postoperative organ dysfunction, preoperative LVEF, emergency operation, Sun's operation, coronary artery bypass grafting, hypertension, cardiac arrhythmia, and advanced avulsion of distal aortic dissection The survival rate was lower, the difference was statistically significant (P<0.05).In addition to OSAHS, coronary artery bypass grafting and preoperative pericardial tamponade were also risk factors for the increase of 36 month mortality rate (HR=11.28,95%CI: 1.98-46.25, P=0.009; HR=9.08, 95%CI: 2.22-41.3, P=0.032). Conclusions: There was no significant difference in mortality during hospitalization in patients with Stanford A aortic dissection combined with OSAHS. The survival rate of 36 months after operation was lower than that of the control group.

Clinical features of 42 cases of COVID-19 / 上海预防医学

Objective To investigate the clinical features, laboratory findings, chest CT findings and treatment of patients with COVID-19, and to analyze their relationship with prognosis. Methods From January to February 2020, the clinical data on the 42 patients with COVID-19 admitted to the Wenzhou Sixth People′s Hospital were analyzed retrospectively. Results The clinical symptoms of the 42 cases included fever (35 cases), cough (26 cases), fatigue (14 cases), aspiration (9 cases), sore throat (4 cases), muscle ache (5 cases), headache (2 cases), nausea (4 cases), diarrhea (6 cases) and abdominal pain (1 case).The absolute number of blood lymphocyte decreased to different degrees in 22 cases.Fourteen cases had lactate dehydrogenase obviously, with no obvious change in procalcitonin.The imaging manifestations were cloud-like and ground-glass-like high density shadows scattered outside the lungs, small flaky consolidation and bronchus inflating sign were seen locally.A few images showed diffuse high density, most of the lesions showed consolidation or striate change, and local fibrosis was formed in the lower lobes of both lungs. Conclusion Fever and cough are the first symptoms of COVID-19, and a few cases are associated with shortness of breath and diarrhea, accompanied by different degrees of systemic symptoms, but most of the patients improve their conditions after active antivirus, anti-infection, systematic symptoms improvement and supportive treatment.The disease is highly infectious and its condition changes rapidly.Therefore, early detection, early diagnosis and comprehensive treatment of the whole body as soon as possible are the keys to treatment.

A prospective multicenter randomized controlled study on the efficacy and safety of pharyngeal spraying recombinant human interferon alpha 2b in the treatment of children with herpangina / 中华实用儿科临床杂志

Objective:To evaluate the efficacy and safety of pharyngeal spraying recombinant human interfe-ron alpha 2b (rhIFNα2b) in the treatment of herpangina in children.Method:s A prospective, multicenter, rando-mized, opened and controlled study was carried out in 11 hospitals in Anhui province from August 2018 to March 2019.According to the time of admission, 180 patients diagnosed as herpangina were prospectively and randomly divided into rhIFNα2b treatment group and Ribavirin control group.On the basis of giving both groups the heat-clearing, detoxifying and anti-infection treatment, the patients in treatment group received pharyngeal spraying rhIFNα2b 9 g/L saline solution[1 million IU/mL, 0.1 million IU/(0.1 mL·press)], and the patients in control group were treated by pharyngeal spraying Ribavirin (0.5 mg RBV/press, 150 press), 3 presses per time, 4 times per day, continuous administration for 5 days for both groups.Those who recovered in advance were no longer given medication.All patients were observed to fully recover.The clinical efficacy and the disappearing time of symptoms and signs between two groups were compared, and the safety of pharyngeal spraying rhIFNα2b for patients was evaluated.Result:s All of the 180 patients completed the study, including 90 cases in the treatment group and 90 cases in the control group.There was no statistically significant difference in terms of gender, age, weight and course of illness before treatment between the two groups (all P>0.05), which had clinical comparability.The apparent efficiency of the treatment group [63.3% (57/90 cases)] was significantly higher than that in the control group [38.9% (35/90 cases)] and the difference was statistically significant( χ2=10.934, P=0.004); no significant difference in the total efficiency between the treatment group [96.7% (87/90 cases)]and the control group [92.2% (83/90 cases)]was observed ( χ2=2.924, P=0.169). The duration of fever[(32.59±20.73) h vs.(45.72±26.96) h], hyperemia[(76.48±23.12) h vs.(92.44±24.31) h], herpes[(72.99±25.77) h vs.(85.09± 26.62) h], salivation[(45.44±24.96) h vs.(54.42±31.20) h] and anorexia[(62.70±23.99) h vs.(78.71±30.54) h] in the treatment group were significantly shorter than those in the control group, and the differences were statistically significant(all P<0.05). Before treatment, the serum levels of tumor necrosis factor α(TNF-α) [(13.02±4.41) ng/L vs.(13.57±9.27) ng/L], interleukin-6(IL-6) [(26.48±11.31) ng/L vs.(30.15±15.55) ng/L] and C-reactive protein(CRP)[(19.34±14.11) mg/L vs.(19.83±14.57) mg/L]were not significantly different between the two groups (all P>0.05). After treatment, the serum levels of TNF-α and IL-6 were(7.26±1.99) ng/L and (2.42±0.73) ng/L in the treatment group, which were significantly lower than those in the control group [(12.09±6.39) ng/L and (7.32±11.51) ng/L](all P<0.05), but no significant difference in serum levels of CRP between the two groups was observed ( P>0.05). The comparison on positive rate of virus in pharyngeal swab between the treatment group [65.3% (32/49 cases) and 40.6% (13/32 cases) respectively] and the control group[66.7%(36/54 cases) and 41.0% (16/39 cases), respectively]before and after therapy showed no significant difference (all P>0.05). During the treatment, no serious adverse reactions were observed in the two groups.The incidence of adverse reactions was 1.1% (1/90 cases) in the treatment group and 5.6% (5/90 cases) in the control group.In addition, the serum hemoglobin level of children in the control group after treatment was significantly lower than that before treatment and that in the treatment group (all P<0.05). Conclusions:Compared with pharyngeal spraying ribavirin, pharyngeal spraying rhIFNα2b can greatly improve the clinical efficiency, accelerate the disappearance of clinical symptoms and signs, and shorten the total course of disease, and is more safe and worthy of clinical application.

Optimization of the Ultrasound Extraction Technology of Jinjuan Shengban Capsules by Response Surface Methodology Combined with Multi-index Comprehensive Scoring / 中国药房

OBJECTIVE： To optimize the ultrasonic extraction technology for Jinjuan shengban capsules. METHODS： Using comprehensive score of indexes as transfer rate of gallic acid， chlorogenic acid， baicalin， aloe emodin and emodin methyl ether， with ethanol volume fraction， ultrasonic power， ultrasonic extraction time and liquid-material ratio as factors， the ultrasound extraction technology of Jinjuan shengban capsules was optimized by Box-Behnken response surface methodology based on single factor test. The validation test was conducted. RESULTS： The best extraction technology was 50-fold 70％ ethanol， extracting 40 min under 300 W. In validation test， average transfer rates of gallic acid， chlorogenic acid， baicalin， aloe emodin and emodin methyl ether were 85.92％， 86.37％， 92.76％， 90.84％ and 87.26％ （RSD＜3.57％，n＝3） in 3 batches of samples； comprehensive score was 88.95％， relative error of which to predicted value of 88.27％ was 1.10％. CONCLUSIONS： The response surface method combined with multi-index comprehensive scoring can be used to optimize the extraction technology of Jinjuan shengban capsules which is simple and stable.

Effects of Different Stimulators on Proliferation of Peripheral Blood Lymphocyte Subsets / 中国实验血液学杂志

OBJECTIVE@#To investigate the effects of different stimultors (PHA, PMA and IL-2) and culture systems (PBMC and whole blood) on the proliferation of human peripheral blood lymphocyte subsets, so as to provide the experimental basis for selecting the appropriate system according to the experimental purposes.@*METHODS@#A total of 10 ml serum samples were collected from healthy volunteers (n=6). The 300 μl whole blood was directly used to detect lymphocyte subsets by flow cytometry. The 400 μl whole blood were inoculated respectively with 3 different stimulators at 37℃ and 5% CO2 for 60 h; Three different stimulators were also added to the PBMC which were isolated from 2 ml whole blood. Then the proliferation ability of lymphocyte subsets was analyzed by flow cytometry.@*RESULTS@#After the PBMC were stimulated with PHA, CD4CD8CD3 lymphocytes were the most subset; The proportion of CD3CD4 T lymphocytes and CD3CD19 B lymphocytes decreased after being stimulated by PMA (P<0.01, P<0.05); the lymphocyte subset ratio had no significant change after being stimulated by IL-2. After the whole blood system was stimulated with PHA, the CD4/CD8 T lymphoblasts were main subsets, the counts of B lymphocytes and NK cells were reduced; after being stimulated with PMA, the number of CD8CD3 T lymphoblast and CD4CD8T lymphocytes increased, the B/NK cells were not distinguished with the surface markers; after the whole blood system was stimulated with IL-2, the proportion of NK cells significantly increased (P<0.05).@*CONCLUSION@#Lymphocyte proliferation stimulated by PMA is the fastest, while the effect of IL-2 on the lymphocyte subset proportion stimulated by IL-2 is the minimal. After being stimulated by PHA the division cycles of lymphocyte are the most.

Effects of soybean isoflavones on the energy metabolism of swimming mice / 中国应用生理学杂志

OBJECTIVE@#To establish an animal model for loaded swimming, so as to investigate the energy metabolism effects of soybean isoflavones (SI) on swimming mice.@*METHODS@#Thirty male Kunming mice were randomly divided into three groups:normal control, swimming group, and swimming+SI group. The normal control group mice were fed a basic AIN-93M diet, the SI groups were supplied with soybean isoflavones(4 g/kg).Two weeks later, the mice were forced to swim for an hour,and then all the mice were killed, the samples of blood, liver and muscles of hind were collected.The serum contents of lactic acid(Lac), the activities of lactic dehydrogenase (LDH), succinate dehydrogenase (SDH), creatine kinase (CK) and ATPase were measured.@*RESULTS@#Compared with normal control,the serum content of Lac was significantly improved in the group of the swimming control and SI(<0.05),the activity of LDH in the serum was obviously improved in the group of the swimming control and SI, and the activity of CK and SDH were both significantly improved in the group of the swimming control and SI except the activity of SDH in the liver of the group SI; compared with the swimming control,the serum contents of Lac,the activities of LDH, ATPase, SDH, CK were obviously improved(<0.05).@*CONCLUSIONS@#Soybean isoflavones can improve the energy metabolism,antioxidant capacity of the swimming mice.

High sensitive ELISA for detection of atrazion / 中国应用生理学杂志

OBJECTIVES@#To set up ELISA for detection of atrazine with high precision.@*METHODS@#The reaction condition of indirect-ELISA was optimized, including atrazine-ovalbumin(AT-OVA) concentration and primary antibody concentration, organic solvent, goat anti-rat immunoglobin G-horseradish peroxidase(IgG-HRP) concentration. The actual samples were detected by the ELISA method established in our laboratory. Then the ELISA method was compared with the HPLC.@*RESULTS@#The specification curve of indirect-ELISA was set up after optimization. The relation coefficient R=0.9958. The limit of detection (LOD) was 1.972 ng/ml. The percent recovery of the actual samples was in range of 80%~120%. The ELISA detection sensitivity was higher than the HPLC in the range of 0 ng/ml~6 ng/ml atrazine.@*CONCLUSIONS@#The ELISA to detect atrazine has good specificity and high precision. And it can be applied in testing real atrazine samples replacing of the large-scale instrument.

Efficient Detection of Environmental Estrogens Bisphenol A and Estradiol By Sensing System Based on AuNP-AuNP-UCNP Triple Structure / 分析化学

A sensing system based on AuNP-AuNP-UCNP triple structure for efficient detection of dual targets was constructed. In the preparation of triple structure, the gold nanoparticles (AuNPs) and upconversion nanoparticles (NaYF4: Yb, Er, Gd, UCNPs) were synthesized and surface modified. Then the two nanoparticles and their aptamers were connected to form two kinds of optical fluorescent probes. A nucleic acid sequence that matches with two aptamers was designed, rendering the probes to get close based on the principle of complementary base pairing. On the basis of this, a sensing system with a triple structure was prepared,and its connecting effect was characterized by TEM. With this system, dual targets of bisphenol A and estradiol were efficiently and conveniently detected through quantitative determination by fluorescence and UV spectrophotometer. At reaction temperature of 30℃ and pH=7.8,this method exhibited good linear range for determination of bisphenol A and estradiol from 2 ng/mL to 200 ng/mL and from 10 ng/mL to 150 ng/mL, with limits of detection of 0.2 ng/mL and 0.5 ng/mL, respectively. This sensing system with the triple structure owned better specificity to structural and functional analogues, and showed good repeatability and stability. What's more,this sensing system was applied in actual water detection,with the recoveries between 86.1% and 107. 4%, and the relative standard deviation below 6. 8%. This method showed promising applications in other environmental estrogens in water samples.

Evaluation of meibomian gland dysfunction after phacoemulsification surgery / 国际眼科杂志(Guoji Yanke Zazhi)

·AIM: To evaluate meibomian gland dysfunction (MGD) after phacoemulsification surgery,and to discuss the potential impact of ocular surface. ·METHODS: A perspective, self-controlled clinical trial was conducted. Fifty-six eyes of 56 patients with MGD underwent phacoemulsification and were evaluated the ocular surface disease index (OSDI), meibography score,lid margin abnormalities, Schirmer test, tear film break - up time ( BUT ) at preoperatively and postoperatively 2wk,1 and 3mo. ·RESULTS: Schirmer test did not change significantly postoperatively ( P > 0. 05 ). The OSDI scores, meibography score, lid margin abnormalities increased at 2wk, 1 and 3mo postoperatively compared to before operation (all P<0.05);BUT decreased (P<0.05). · CONCLUSION: Meibomian gland function may be aggravated after phacoemulsification surgery.

Clinical observation of scleral buckling with 25 G for retinal detachment eyes with silicon oil tamponaded / 国际眼科杂志(Guoji Yanke Zazhi)

AIM: To observe the effect of 25G- assisted scleral buckling under direct vision in the treatment of retinal detachment in silicone oil-filled eyes.?METHODS: In the retrospective case study, 15 eyes of 15 patients who had retinal detachment after silicone oil filling were treated with 25G- assisted scleral buckling under direct vision. Experimental patients were followed up for 3-6mo to observe the retinal reattachment, visual acuity and intraocular pressure.?RESULTS: There were 15 eyes of 15 patients with inferior retinal detachment before operation. Retinal reattachment was achieved in 13 eyes (13 patients), and retina failed to be reattached in 2 eyes ( 2 patients ) after operation. Furthermore, postoperative visual acuity was found to be improved more than one line in 11 eyes ( 11 patient ) , unchanged in 3 eyes ( 3 patients ) , and decreased more than one line in 1 eye ( 1 patient ) . Meanwhile, early postoperative intraocular pressure increased in some patients and returned to normal after drug control.?CONCLUSION:The 25G-assisted scleral buckling under direct vision has a definite curative effect in the treatment of retinal detachment in silicone oil-filled eyes.

Effect of cordycepin on apoptosis and autophagy of tongue cancer cells in vitro and the molecular mechanism / 南方医科大学学报

<p><b>OBJECTIVE</b>To study the effect of cordycepin on cell cycle, apoptosis and autophagy of human tongue cancer TCA-8113 cells and explore the mechanism of cordycepin for inhibiting the occurrence of tongue cancer.</p><p><b>METHODS</b>CCK-8 method was used to assess the inhibitory effect of cordycepin on TCA-8113 cell proliferation in vitro. The cell cycle and cell apoptosis of TCA-8113 cells treated with different concentrations of cordycepin were analyzed using flow cytometry. The expressions of apoptosis-related genes caspase-3, caspase-9, Bcl-2, and Bax were examined using quantitative real-time PCR and Western blotting, and immunohistochemistry was used to detect the expressions of autophagy-related proteins LC-3β, P62, p-mTOR, and AMPK.</p><p><b>RESULTS</b>CCK-8 assay showed that cordycepin significantly inhibited the proliferation of TCA-8113 cells in a concentration-dependent manner with an IC of 3.548 mg/mL at 24 h and an IC of 1.185 mg/mL at 48 h. Flow cytometric analysis showed that cordycepin caused cell cycle arrest at S phase and dose-dependently increased the apoptotic rate of TCA-8113 cells. Treatment of the cells with cordycepin enhanced the expressions of Bax, caspase-3 and caspase-9 at both the mRNA and protein levels and inhibited the expression of the antiapoptotic gene Bcl-2. Immunohistochemistry demonstrated that cordycepin promoted the expression of LC-3β and AMPK and inhibited the expression of P62 and p-mTOR.</p><p><b>CONCLUSION</b>Cordycepin inhibits the proliferation and induces apoptosis of HCT-116 cells through the mitochondrial pathway and induces autophagy via the AMPK/mTOR pathway.</p>

The comparation of establishing the allergic rhinitis model between Kunming mice and BALB/c mice / 中华耳鼻咽喉头颈外科杂志

Objective@#To discusses the feasibility of establishing the allergic rhinitis (AR) model in Kunming mice.@*Methods@#Kunming mice (n=20) and BALB/c mice (n=20) were sensitized and motivated by ovalbumin (OVA) and aluminium hydroxide. The symptoms rating scale (symptoms score>5 as successful model) was used to evaluate AR symptoms of two kinds of mice after the AR model were set up. The differences of OVA serum specific IgE (OVA-sIgE) and the levels of interleukin 4 (IL-4) in Kunming mice model group and Kunming mice blank group were evaluated, the symptom scores, OVA serum specific IgE (OVA-sIgE) and the levels of interleukin 4 (IL-4) of successful model of Kunming mice and BALB/c mice were compared, and the difference between two kinds of animal models. SPSS 22.0 statistical software was used for statistical analysis was analysed. SPSS 22.0 statistical software was used for statistical analysis.@*Results@#During the experiment, 2 mice died in BALB/c mouse model group, and no mouse died in Kunming mouse model group. Kunming model group gained a certain incease of weight. The clinical symptom scores, OVA - sIgE levels, serum IL - 4 levels of Kunming mouse model group were significantly higher than those of blank control group. The difference was statistically significant [(6.100±0.568) vs (2.700±0.823), (29.083±10.470) ng/ml vs (3.908±1.984) ng/ml, (219.250±30.821) pg/ml vs (140.056±27.684) pg/ml, all P<0.05]. Compared with BALB/c mice model group, the symptom score and serum level of IL-4 of Kunming mice model group had no statistically significant differences (6.100±0.568) vs (6.313±0.704), (219.250±30.821) pg/ml vs (253.294±53.953) pg/ml, all P>0.05), and the difference of serum level of OVA-sIgE was statistically significant ((29.083±10.470) ng/ml vs (76.277±25.724) ng/ml, P<0.05).@*Conclusions@#Kunming mice are able to obtain a clinically significant AR model through OVA modeling, with a significant increase in serum OVA-SIgE and IL-4. Kunming mice AR model shows good tolerance, and could reduce the losses in animal experiment.

Isolation and characterization of antimicrobial peptides from healthy male urine

A complex of low-molecular cationic peptides, extracted from human urine by a combination membrane ultrafiltration and weak cation exchange chromatography, was characterized in this study. It provides a simpler solution for the development of novel antimicrobial peptides from biological liquid waste

Comparative analysis of emmetropia biology measurement in Han and Kazak college students / 国际眼科杂志(Guoji Yanke Zazhi)

? AlM: To observe the differences of central cornea thickness, anterior chamber depth, corneal anterior curvature and corneal posterior curvature between the Kazak and the Han nationality college emmetropia students, and analyze the relationship of corneal thickness and corneal curvature.?METHODS: More than 500 students in grade one in Xinjiang Medical University for screening, selected 55 emmetropia eyes in Han nationality students and 51 in Kazak students. Sirius corneal topography was applied to the measurement.? RESULTS: Kazak and the Han nationality college emmetropia students' central cornea thickness ( Kazak:0. 52± 0. 03mm, Han:0. 54 ± 0. 03mm), anterior chamber depth (Kazak:2. 97 ± 0. 31mm, Han: 3. 14 ± 0. 25mm) had significant difference (P0. 05). Han had no significant relationship in corneal thickness and corneal anterior curvature (r<0), or in corneal posterior curvature ( r < 0. 1 ) . Kazak had no significant relationship in corneal thickness and corneal anterior curvature (r<0), or in corneal posterior curvature(r<0. 1).? CONCLUSlON: There are significant differences between the Kazak and the Han nationality college emmetropia students in cornea thickness, anterior chamber depth. There is no significant relationship between corneal thickness and corneal curvature (K1, K2) in Kazak and the Han nationality college emmetropia students.

Purification of monoclonal antibody to clenbuterol and its biology identity / 中国应用生理学杂志

<p><b>OBJECTIVE</b>To identify the self-preparation monoclonal antibody which target to clenbuterol, and set up the standard curve to clenbuterol (CL) detection.</p><p><b>METHODS</b>The affinity constants and activity of the monoclonal antibody which target to CL were determined by ELISA. ELISA was also used to confirm whether the monoclonal antibody had any across-reaction with BSA and CL analogues. The rat ascites which contains the monoclonal antibody target to CL was purified by (NH4)2SO4 salt-out method and further by affinity column. At last, the CL detection standard curve which based on indirect competition ELISA was established.</p><p><b>RESULTS</b>The ELISA experiment showed that the antibody titer was 10(6) and the monoclonal antibody affinity constants was 2.90 x 10(10) L/mol. The result of the indirect competition ELISA confirmed that the monoclonal antibody had no cross-reaction with BSA and a few kind of CL analogue. CL detection standard curve based on indirect competition ELISA was established, which R2 was 0.9812, and the lowest detectable limit was 1.0 ng/ml.</p><p><b>CONCLUSION</b>The standard curve based on indirectly competitioning ELISA was established. The self-preparation monoclonal antibody which target to CL has high affinity and high specific to CL, which had established the foundation to the advanced development of the CL immune test paper and CL ELISA kit.</p>